rabbit anti ck 18 antibody  (Bioss)

Journal: Materials Today Bio

Article Title: In situ vascularization and epithelialization of segmental bioengineered trachea based on marrow-derived stem/progenitor cells

doi: 10.1016/j.mtbio.2025.101990

Figure Lengend Snippet: Morphological and staining analysis of MSCs after differentiation into epithelial-like cells. Representative images include optical microscopy, H&E staining, and immunofluorescence staining for CK5/CK18 in undifferentiated MSCs, differentiated MSCs (MSCs to Epi), and primary tracheal epithelial cells (positive control). A-C Scale bars = 100 μm, D Scale bars = 400 μm, ∗, P < 0.05; ∗∗, P < 0.01.

Article Snippet: Cells were incubated overnight at 4 °C with Rabbit Anti-CK18 antibody (1:200, bs-2043R, Bioss, China) and Rabbit Anti-CK5 antibody (1:200, bs-1060R, Bioss, China).

Techniques: Staining, Microscopy, Immunofluorescence, Positive Control

Journal: Materials Today Bio

Article Title: In situ vascularization and epithelialization of segmental bioengineered trachea based on marrow-derived stem/progenitor cells

doi: 10.1016/j.mtbio.2025.101990

Figure Lengend Snippet: Histological analysis of tracheal graft specimens from experimental animals surviving for more than 6 months. (A–D) H&E staining. (E–H) MT staining. (I–L) Immunofluorescence staining for αSMA. (M − P) Immunofluorescence for CK-18. The area marked with an asterisk (∗) corresponds to the location of PCL. Quantitative analysis of immunofluorescence staining (Q) α-SMA-positive microvessels and (R) CK18-positive epithelial coverage were quantified in three randomly selected high-power fields per graft section. A,C,E,G Scale bars = 500 μm; B,D,F,H Scale bars = 50 μm; I,K,M,O Scale bars = 400 μm; J,L,N,P Scale bars = 100 μm. n: not significant.

Article Snippet: Cells were incubated overnight at 4 °C with Rabbit Anti-CK18 antibody (1:200, bs-2043R, Bioss, China) and Rabbit Anti-CK5 antibody (1:200, bs-1060R, Bioss, China).

Techniques: Staining, Immunofluorescence

Journal: PLoS ONE

Article Title: A New Method to Isolate and Culture Rat Kupffer Cells

doi: 10.1371/journal.pone.0070832

Figure Lengend Snippet: The results indicated that contaminating cells detected both in P0 and P3 cell cultures were few and included SECs (positive for CD31) and HSC (positive for alpha-SMA), but not hepatocytes (positive for CK18). (Blue: DAPI, Green: FITC beads).

Article Snippet: After 1 day of culture, the slides were rinsedwith PBS and fixed with 4% paraformaldehyde at room temperature for 15 min. After permeabilization with 0.1% Triton X-100 for 10 min followed by blocking with 10% normal goat serum for 30 min, the cells were incubated with the following antibodies overnight at 4°C: rabbit polyclonal anti-CD68 (ED-1, Bioss, China), rabbit polyclonal anti-CD163 (ED-2, Bioss, China), rabbit polyclonal anti-CK18 (Bioss, China), rabbit polyclonal anti-CD31 (Bioss, China) and rabbit polyclonal anti- alpha-SMA (Santa Cruz, USA).

Techniques: